pLV-EF1A-CD19.28H.28TM.28.3z vector (V018010)

LOCUS       .                       8753 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        3..229
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     misc_feature    457..582
                     /label="HIV-1 Ψ"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1075..1308
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /gene="Protein Tat"
                     /label="Protein Tat"
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     misc_feature    1803..1920
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /label="attB4"
                     /note="core recombination site for the Gateway® BP
                     reaction"
     promoter        1965..2176
                     /label="EF-1α core promoter"
                     /note="core promoter for human elongation factor EF-1α"
     protein_bind    2182..2206
                     /label="attB1"
                     /note="recombination site for the Gateway® BP reaction"
     CDS             3830..4543
                     /label="EGFP"
                     /note="the original enhanced GFP (Yang et al., 1996)"
     protein_bind    complement(4556..4580)
                     /label="attB2"
                     /note="recombination site for the Gateway® BP reaction"
     misc_feature    4598..5186
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             5274..5507
                     /label="3' LTR (ΔU3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5579..5713
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      5740..5875
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(5896..5914)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5924..5940)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      6082..6537
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6563..6667
                     /label="AmpR promoter"
     CDS             6668..7525
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      7699..8287
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    8575..8596
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8611..8641
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    8649..8665
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     primer_bind     8673..8689
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        8710..8728
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"