Basic Vector Information
- Vector Name:
- pPEgRNA
- Antibiotic Resistance:
- Ampicillin
- Length:
- 2564 bp
- Type:
- Gene-editing vector
- Replication origin:
- ori
- Host:
- Bacteria
- Promoter:
- J23119
- 3' Primer:
- AmpNR
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pPEgRNA vector Map
pPEgRNA vector Sequence
LOCUS . 2564 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
terminator complement(join(2540..2564,1..19))
/label="bacterial terminator"
/note="putative bacterial transcription terminator"
promoter 37..71
/label="J23119(SpeI) promoter"
/note="bacterial promoter (Registry of Standard Biological
Parts BBa_J23119) modified to end with an SpeI site"
misc_RNA 72..147
/label="gRNA scaffold"
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
CDS 168..197
/label="Myc"
/note="Myc (human c-Myc proto-oncogene) epitope tag"
CDS 213..230
/label="6xHis"
/note="6xHis affinity tag"
terminator 456..502
/label="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
rep_origin complement(726..1314)
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1488..2345)
/label="AmpR"
/note="β-lactamase"
promoter complement(2346..2450)
/label="AmpR promoter"
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