AKT-translocation-mStrawberry reporter vector (V018005)

LOCUS       .                      10887 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        1..212
                     /label="EF-1α core promoter"
                     /note="core promoter for human elongation factor EF-1α"
     intron          213..1151
                     /label="EF-1α intron A"
                     /note="intron upstream of the start codon of human EF-1α"
     CDS             3153..3860
                     /label="mStrawberry"
                     /note="monomeric derivative of DsRed fluorescent protein"
     misc_feature    3905..4022
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        4077..4581
                     /label="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             4593..4988
                     /label="BSD"
                     /note="blasticidin S deaminase"
     misc_feature    5024..5612
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             5684..5917
                     /label="3' LTR (ΔU3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5995..6129
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      6156..6291
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(6312..6330)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      6498..6953
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6979..7083
                     /label="AmpR promoter"
     CDS             7084..7941
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      8115..8703
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    8991..9012
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9027..9057
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     promoter        9126..9144
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9172..9398
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9399..9579
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9626..9751
                     /label="HIV-1 Ψ"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     promoter        10000..11160
                     /label="EF-1α promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1α"