Basic Vector Information
- Vector Name:
- pPPARγ-Fluc-SV40-hRluc-Neo
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6949 bp
- Type:
- Transcription factor reporter
- Replication origin:
- ori
- Host:
- Mammalian cells
- Selection Marker:
- Neo/G418
- Promoter:
- PPARγ
- 5' Primer:
- cer region-F1
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pPPARγ-Fluc-SV40-hRluc-Neo vector Map
pPPARγ-Fluc-SV40-hRluc-Neo vector Sequence
LOCUS . 6949 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
polyA_signal complement(115..236)
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
promoter 481..838
/label="SV40 promoter"
/note="SV40 enhancer and early promoter"
CDS 889..1821
/label="hRluc"
/note="Renilla luciferase"
CDS 1873..2661
/label="NeoR/KanR"
/note="aminoglycoside phosphotransferase"
polyA_signal 2728..2776
/label="poly(A) signal"
/note="synthetic polyadenylation signal"
promoter 2926..3030
/label="AmpR promoter"
CDS 3031..3888
/label="AmpR"
/note="β-lactamase"
rep_origin complement(4049..4637)
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 4753..5036
/label="cer region"
/note="ColE1-derived recombination site that helps to
maintain plasmids as monomers"
promoter 5202..5233
/label="minP"
/note="minimal TATA-box promoter with low basal activity"
CDS 5244..6893
/label="luciferase"
/note="firefly luciferase"
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