Basic Vector Information
- Vector Name:
- pT7-PE7 for IVT
- Antibiotic Resistance:
- Ampicillin
- Length:
- 10552 bp
- Type:
- Gene-editing vector
- Replication origin:
- ori
- Host:
- E. coli
- Promoter:
- T7
- 5' Primer:
- CMV-F
- Growth Strain(s):
- Stable
- Growth Temperature:
- 37℃
pT7-PE7 for IVT vector Map
pT7-PE7 for IVT vector Sequence
LOCUS . 10552 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
promoter 1..204
/label="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 340..360
/label="SV40 NLS"
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
CDS 358..4461
/label="Cas9(H840A)"
/note="nickase mutant of the Cas9 endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system"
CDS 4519..4539
/label="SV40 NLS"
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
CDS 7318..7338
/label="SV40 NLS"
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
CDS 7348..7374
/label="c-myc NLS"
/note="nuclear localization signal of human c-Myc
proto-oncogene (Dang and Lee, 1988)"
polyA_signal 7482..7706
/label="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
promoter complement(7825..7855)
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind complement(7870..7891)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(8179..8767)
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(8941..9798)
/label="AmpR"
/note="β-lactamase"
promoter complement(9799..9903)
/label="AmpR promoter"
enhancer 10173..10552
/label="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
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