Basic Vector Information
- Vector Name:
- TRUPATH Triple Gai1
- Antibiotic Resistance:
- Ampicillin
- Length:
- 10159 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells
- Selection Marker:
- Neo/G418
- Promoter:
- CMV
- 5' Primer:
- CMV-F
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
TRUPATH Triple Gai1 vector Map
TRUPATH Triple Gai1 vector Sequence
LOCUS . 10159 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
enhancer 235..614
/label="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 863..881
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 1997..2713
/label="EGFP"
/note="the original enhanced GFP (Yang et al., 1996)"
misc_feature 3036..3614
/label="IRES2"
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
CDS 3899..4831
/label="Green Renilla Luciferase"
/note="green-emitting variant of Renilla luciferase"
polyA_signal 5756..5980
/label="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
rep_origin 6026..6454
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 6468..6797
/label="SV40 promoter"
/note="SV40 enhancer and early promoter"
CDS 6864..7655
/label="NeoR/KanR"
/note="aminoglycoside phosphotransferase"
polyA_signal 7832..7965
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
promoter complement(8050..8080)
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind complement(8095..8116)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(8404..8992)
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(9166..10023)
/label="AmpR"
/note="β-lactamase"
promoter complement(10024..10128)
/label="AmpR promoter"
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