Basic Vector Information
- Vector Name:
- CMV-Cas13X.1-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7658 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells
- Promoter:
- CMV, U6
- 5' Primer:
- U6-F
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
CMV-Cas13X.1-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA vector Map
CMV-Cas13X.1-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA vector Sequence
LOCUS . 7658 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
intron 139..366
/label="hybrid intron"
/note="hybrid between chicken β-actin (CBA) and minute
virus of mice (MMV) introns (Gray et al., 2011)"
CDS 387..407
/label="SV40 NLS"
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
CDS 2730..2750
/label="SV40 NLS"
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
polyA_signal 2760..2894
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
promoter 2901..3141
/label="U6 promoter"
/note="RNA polymerase III promoter for human U6 snRNA"
enhancer 3291..3594
/label="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 3595..3798
/label="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 3833..4540
/label="mCherry"
/note="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
polyA_signal 4560..4784
/label="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
promoter 5206..5310
/label="AmpR promoter"
CDS 5311..6168
/label="AmpR"
/note="β-lactamase"
rep_origin 6342..6930
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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