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pArk313 vector (V017994)

Basic Vector Information

Vector Name:
pArk313
Antibiotic Resistance:
Ampicillin
Length:
6088 bp
Type:
Protein expression
Replication origin:
ori
Host:
Adeno-associated virus
Promoter:
P5
3' Primer:
AmpNR
Growth Strain(s):
Stable
Growth Temperature:
37℃

pArk313 vector Map

Copy Sequence View Map
pArk3136088 bp30060090012001500180021002400270030003300360039004200450048005100540057006000Rep78WELQut siteoriAmpRAmpR promoter

pArk313 vector Sequence

LOCUS       .                       6088 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             149..2005
                     /gene="Rep78"
                     /label="Rep78"
                     /note="Protein Rep78 from Adeno-associated virus 2 (isolate
                     Srivastava/1982). Accession#: Q89268"
     CDS             4077..4088
                     /label="WELQut site"
                     /note="WELQut protease recognition and cleavage site"
     rep_origin      complement(4339..4927)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5101..5958)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(5959..6063)
                     /label="AmpR promoter"

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