pAf-CRISPR-phoA vector (V017993)

LOCUS       .                      13733 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_RNA        3129..3204
                     /label="gRNA scaffold"
                     /note="guide RNA scaffold for the Streptococcus pyogenes
                     CRISPR/Cas9 system"
     CDS             complement(3645..7748)
                     /label="Cas9"
                     /note="S. pyogenes Cas9, codon optimized for C. elegans
                     with synthetic introns"
     CDS             complement(7779..7799)
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     intron          complement(7820..7935)
                     /label="gpdA intron"
                     /note="intron from the Aspergillus nidulans
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     promoter        complement(9104..9134)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(9149..9170)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(9458..10046)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(10220..11077)
                     /label="AmpR"
                     /note="β-lactamase"
     gene            11095..13102
                     /label="ptrA"
                     /note="useful as a dominant selectable marker in
                     Aspergillus"