Basic Vector Information
- Vector Name:
- pAAV-CAG-TagBFP-HBV-caspase3-TEVp
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7626 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells, Adeno-associated virus
- Promoter:
- CAG
- 3' Primer:
- SV40-R2
- Growth Strain(s):
- Stbl3
- Growth Temperature:
- 37℃
pAAV-CAG-TagBFP-HBV-caspase3-TEVp vector Map
pAAV-CAG-TagBFP-HBV-caspase3-TEVp vector Sequence
LOCUS . 7626 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
rep_origin complement(182..770)
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(944..1801)
/label="AmpR"
/note="β-lactamase"
promoter complement(1802..1906)
/label="AmpR promoter"
rep_origin complement(1932..2387)
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2899..3176
/label="chicken β-actin promoter"
CDS 3597..4295
/label="TagBFP"
/note="monomeric blue fluorescent protein"
misc_RNA 4394..4412
/label="MS2 stem loop"
/note="stem loop that binds the bacteriophage MS2 coat
protein"
CDS 4827..4880
/label="T2A"
/note="2A peptide from Thosea asigna virus capsid protein"
CDS 5418..5438
/label="TEV site"
/note="tobacco etch virus (TEV) protease recognition and
cleavage site"
CDS 5808..6509
/label="TEV protease"
/note="tobacco etch virus protease (Kapust et al., 2001)"
misc_feature 6528..7116
/label="WPRE"
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
polyA_signal complement(7159..7280)
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
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