Basic Vector Information
- Vector Name:
- pGEX-HRV3C-6×His
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5511 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- E. coli
- Copy Number:
- Low
- Promoter:
- Tac
- 5' Primer:
- pGEX-5'
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
- Expression Method:
- IPTG induction
pGEX-HRV3C-6×His vector Map
pGEX-HRV3C-6×His vector Sequence
LOCUS . 5511 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
promoter 183..211
/label="tac promoter"
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
protein_bind 219..235
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG)."
CDS 258..911
/label="GST"
/note="glutathione S-transferase from Schistosoma
japonicum"
CDS 918..935
/label="thrombin site"
/note="thrombin recognition and cleavage site"
CDS 1482..1499
/label="6xHis"
/note="6xHis affinity tag"
promoter 1814..1918
/label="AmpR promoter"
CDS 1919..2776
/label="AmpR"
/note="β-lactamase"
rep_origin 2950..3538
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 3782..3859
/label="lacIq promoter"
/note="In the lacIq allele, a single base change in the
promoter boosts expression of the lacI gene about 10-fold."
CDS 3860..4939
/label="lacI"
/note="lac repressor"
protein_bind 4955..4976
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 4991..5021
/label="lac promoter"
/note="promoter for the E. coli lac operon"
CDS 5065..5238
/label="lacZα"
/note="LacZα fragment of β-galactosidase"
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