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pDG148-Cre vector (V017960)

Basic Vector Information

Vector Name:
pDG148-Cre
Antibiotic Resistance:
Ampicillin
Length:
9292 bp
Type:
Protein expression
Replication origin:
ori
Host:
Bacillus subtilis
Growth Strain(s):
DH5a
Growth Temperature:
37℃

pDG148-Cre vector Map

Copy Sequence View Map
pDG148-Cre9292 bp400800120016002000240028003200360040004400480052005600600064006800720076008000840088009200lac operatorCrelacIbomoriAmpRAmpR promoterrepB

pDG148-Cre vector Sequence

LOCUS       .                       9292 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     protein_bind    270..286
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     CDS             310..1338
                     /label="Cre"
                     /note="site-specific recombinase"
     CDS             1590..2669
                     /label="lacI"
                     /note="lac repressor"
     misc_feature    2843..2983
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(3169..3757)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3931..4788)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(4789..4893)
                     /label="AmpR promoter"
     CDS             5412..6413
                     /label="repB"
                     /note="RepB replication protein"

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