pT3-myr-AKT-HA-IRES-Fluc vector (V017949)

LOCUS       .                       9385 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        1..1179
                     /label="EF-1α promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1α"
     promoter        1196..1214
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1293..1317
                     /label="attB1"
                     /note="recombination site for the Gateway® BP reaction"
     CDS             1338..1358
                     /label="myr"
                     /note="N-myristoylation signal from Src kinase (Pellman et
                     al., 1985; Kaplan et al., 1988)"
     CDS             1371..2810
                     /label="Akt1"
                     /note="human serine/threonine protein kinase activated by
                     growth factors"
     CDS             2811..2837
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     protein_bind    complement(2857..2881)
                     /label="attB2"
                     /note="recombination site for the Gateway® BP reaction"
     CDS             2934..2975
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     misc_feature    2998..3574
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             3575..5224
                     /label="luciferase"
                     /note="firefly luciferase"
     CDS             5228..5269
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     polyA_signal    5313..5537
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    5607..5640
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6098..6114)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        6588..6692
                     /label="AmpR promoter"
     CDS             6693..7550
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      7724..8312
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    8600..8621
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8636..8666
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    8674..8690
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     primer_bind     8698..8714
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        9387..10564
                     /label="EF-1α promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1α"