pT3-U6-shRNA-Negative-Control-EF1a-myr-AKT-HA vector (V017941)

LOCUS       .                       7402 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        1..1179
                     /label="EF-1α promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1α"
     promoter        1196..1214
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1293..1317
                     /label="attB1"
                     /note="recombination site for the Gateway® BP reaction"
     CDS             1338..1358
                     /label="myr"
                     /note="N-myristoylation signal from Src kinase (Pellman et
                     al., 1985; Kaplan et al., 1988)"
     CDS             1371..2810
                     /label="Akt1"
                     /note="human serine/threonine protein kinase activated by
                     growth factors"
     CDS             2811..2837
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     protein_bind    complement(2857..2881)
                     /label="attB2"
                     /note="recombination site for the Gateway® BP reaction"
     CDS             2934..2975
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     polyA_signal    3019..3243
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    3313..3346
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(3804..3820)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        4294..4398
                     /label="AmpR promoter"
     CDS             4399..5256
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      5430..6018
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    6306..6327
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6342..6372
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    6380..6396
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     primer_bind     6404..6420
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        6890..7130
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     promoter        7404..8581
                     /label="EF-1α promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1α"