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pTK-Nluc vector (V017940)

Basic Vector Information

Vector Name:
pTK-Nluc
Antibiotic Resistance:
Ampicillin
Length:
3625 bp
Type:
Luciferase reporter
Replication origin:
ori
Host:
Mammalian cells
Promoter:
TK
5' Primer:
chimeric intron-F
Growth Strain(s):
DH5a
Growth Temperature:
37℃

pTK-Nluc vector Map

Copy Sequence View Map
pTK-Nluc3625 bp60012001800240030003600HSV TK promoterchimeric intronT7 promoterNlucSV40 poly(A) signalAmpR promoterAmpRori

pTK-Nluc vector Sequence

LOCUS       .                       3625 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        8..759
                     /label="HSV TK promoter"
                     /note="herpes simplex virus thymidine kinase promoter"
     intron          829..961
                     /label="chimeric intron"
                     /note="chimera between introns from human β-globin and
                     immunoglobulin heavy chain genes"
     promoter        1006..1024
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1034..1546
                     /label="Nluc"
                     /note="NanoLuc® luciferase"
     polyA_signal    complement(1580..1701)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        1834..1938
                     /label="AmpR promoter"
     CDS             1939..2796
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      2970..3558
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"

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