Basic Vector Information
- Vector Name:
- pC0054-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q-T375G)-CMV-EGFP
- Antibiotic Resistance:
- Ampicillin
- Length:
- 11387 bp
- Type:
- Gene-editing vector
- Replication origin:
- ori
- Host:
- Mammalian cells
- Promoter:
- CMV
- 3' Primer:
- BGH-R
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pC0054-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q-T375G)-CMV-EGFP vector Map
pC0054-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q-T375G)-CMV-EGFP vector Sequence
LOCUS . 11387 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
promoter 154..483
/label="SV40 promoter"
/note="SV40 enhancer and early promoter"
CDS 550..1341
/label="NeoR/KanR"
/note="aminoglycoside phosphotransferase"
polyA_signal 1518..1651
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
promoter complement(1736..1766)
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind complement(1781..1802)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(2090..2678)
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2852..3709)
/label="AmpR"
/note="β-lactamase"
promoter complement(3710..3814)
/label="AmpR promoter"
enhancer 4080..4459
/label="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 4460..4663
/label="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 4708..4726
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 8050..8076
/label="NES"
/note="nuclear export signal from the HIV Rev protein
(Fischer et al., 1995)"
promoter 9659..9862
/label="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 10046..10762
/label="EGFP"
/note="the original enhanced GFP (Yang et al., 1996)"
polyA_signal 10829..11053
/label="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
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