pRK5-FLAG-SZT2 vector (V017935)

LOCUS       .                      14846 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        1..380
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        815..833
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             924..947
                     /label="FLAG"
                     /note="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
     polyA_signal    11146..11280
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        11349..11706
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      11955..12410
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        12692..12796
                     /label="AmpR promoter"
     CDS             12797..13654
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      13828..14416
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    14704..14725
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        14740..14770
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    14778..14794
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     primer_bind     14802..14818
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"