pMSGV-mCD105scFv-myc-CD28HTMcostim-T2A-Thy1.1 vector (V017933)

LOCUS       .                       7547 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..517
                     /label="5' LTR"
                     /note="5' long terminal repeat from murine embryonic stem
                     cell virus"
     CDS             1020..1427
                     /label="gag (truncated)"
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
     misc_feature    1437..1807
                     /label="pol region"
                     /note="Moloney murine leukemia virus (MMLV) pol region
                     containing the splice acceptor site"
     CDS             1909..1938
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             3319..3372
                     /label="T2A"
                     /note="2A peptide from Thosea asigna virus capsid protein"
     CDS             3373..3858
                     /label="Thy1.1"
                     /note="mouse GPI-anchored cell surface glycoprotein of the
                     immunoglobulin superfamily"
     LTR             3932..4446
                     /label="3' LTR"
                     /note="3' long terminal repeat from murine embryonic stem
                     cell virus"
     protein_bind    complement(4618..4634)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4642..4672)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4687..4708)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4996..5584)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5758..6615)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(6616..6720)
                     /label="AmpR promoter"