Basic Vector Information
- Vector Name:
- pBbS5a-Opto-Cre-Vvd-2
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7126 bp
- Type:
- Protein expression
- Replication origin:
- pSC101 ori
- Host:
- Bacteria
- Copy Number:
- Low
- Promoter:
- lacUV5
- 5' Primer:
- LacI-R
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pBbS5a-Opto-Cre-Vvd-2 vector Map
pBbS5a-Opto-Cre-Vvd-2 vector Sequence
LOCUS . 7126 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
promoter 119..149
/label="lac UV5 promoter"
/note="E. coli lac promoter with an 'up' mutation"
protein_bind 157..173
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG)."
CDS 379..828
/label="pMag"
/note="'positive Magnet' variant of an N-terminally
truncated Vivid (VVD) blue light photoreceptor from
Neurospora crassa (Kawano et al., 2015)"
CDS 887..1336
/label="pMag"
/note="'positive Magnet' variant of an N-terminally
truncated Vivid (VVD) blue light photoreceptor from
Neurospora crassa (Kawano et al., 2015)"
terminator 2254..2325
/label="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 2341..2368
/label="T7Te terminator"
/note="phage T7 early transcription terminator"
CDS complement(2902..3849)
/label="Rep101"
/note="RepA protein needed for replication with the pSC101
origin"
rep_origin complement(3897..4119)
/label="pSC101 ori"
/note="low-copy replication origin that requires the Rep101
protein"
terminator complement(4611..4705)
/label="lambda t0 terminator"
/note="transcription terminator from phage lambda"
CDS complement(4738..5595)
/label="AmpR"
/note="β-lactamase"
promoter complement(5596..5700)
/label="AmpR promoter"
protein_bind complement(5740..5761)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
CDS complement(5777..6856)
/label="lacI"
/note="lac repressor"
promoter complement(6857..6934)
/label="lacI promoter"
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