Basic Vector Information
- Vector Name:
- pCRE-miniP-Fluc-hPEST-Neo
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6130 bp
- Type:
- Transcription factor reporter
- Replication origin:
- ori
- Host:
- Mammalian cells
- Selection Marker:
- Neo/G418
- Promoter:
- CRE
- 5' Primer:
- SV40pro-F
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pCRE-miniP-Fluc-hPEST-Neo vector Map
pCRE-miniP-Fluc-hPEST-Neo vector Sequence
LOCUS . 6130 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
promoter 151..182
/label="minP"
/note="minimal TATA-box promoter with low basal activity"
CDS 215..1864
/label="luciferase"
/note="firefly luciferase"
CDS 1868..1987
/label="hPEST"
/note="PEST degradation sequence from mouse ornithine
decarboxylase"
polyA_signal complement(2039..2160)
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
promoter 2354..2711
/label="SV40 promoter"
/note="SV40 enhancer and early promoter"
CDS 2778..3569
/label="NeoR/KanR"
/note="aminoglycoside phosphotransferase"
polyA_signal 3746..3879
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(4224..4812)
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5015..5872)
/label="AmpR"
/note="β-lactamase"
polyA_signal 5977..6025
/label="poly(A) signal"
/note="synthetic polyadenylation signal"
misc_feature 6039..6130
/label="pause site"
/note="RNA polymerase II transcriptional pause signal from
the human α2 globin gene"
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