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pHH21-BNA-Luc vector (V017916)

Basic Vector Information

Vector Name:
pHH21-BNA-Luc
Antibiotic Resistance:
Ampicillin
Length:
4631 bp
Type:
Luciferase reporter
Replication origin:
ori
Host:
Mammalian cells
Selection Marker:
Fluc
Promoter:
RNA polymease I
3' Primer:
AmpNR
Growth Strain(s):
DH5a
Growth Temperature:
37℃

pHH21-BNA-Luc vector Map

Copy Sequence View Map
pHH21-BNA-Luc4631 bp600120018002400300036004200luciferaserrnB T1 terminatorrrnB T2 terminatorAmpR promoterAmpRori

pHH21-BNA-Luc vector Sequence

LOCUS       .                       4631 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             complement(520..2169)
                     /label="luciferase"
                     /note="firefly luciferase"
     terminator      2601..2687
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2779..2806
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        2825..2916
                     /label="AmpR promoter"
     CDS             2917..3774
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      3948..4536
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"

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