pNFAT-pTA-GFP-Neo vector (V017910)

LOCUS       .                       4804 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        177..208
                     /label="minP"
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             241..954
                     /label="GFP (S65T)"
                     /note="S65T variant of Aequorea victoria green fluorescent
                     protein (Heim et al., 1995)"
     polyA_signal    complement(1001..1122)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        1316..1673
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             1704..2495
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    2523..2571
                     /label="poly(A) signal"
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(2898..3486)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3689..4546)
                     /label="AmpR"
                     /note="β-lactamase"
     misc_feature    4713..4804
                     /label="pause site"
                     /note="RNA polymerase II transcriptional pause signal from
                     the human α2 globin gene"