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pBiT2.1-N[TK-SmBiT-Strep-TagII] vector (V017898)

Basic Vector Information

Vector Name:
pBiT2.1-N[TK-SmBiT-Strep-TagII]
Antibiotic Resistance:
Ampicillin
Length:
3402 bp
Type:
Protein expression
Replication origin:
ori
Host:
Mammalian cells
Promoter:
TK
3' Primer:
SV40-R2
Growth Strain(s):
DH5a
Growth Temperature:
37℃

pBiT2.1-N[TK-SmBiT-Strep-TagII] vector Map

Copy Sequence View Map
pBiT2.1-N[TK-SmBiT-Strep-TagII]3402 bp6001200180024003000HSV TK promoterStrep-Tag IISV40 poly(A) signaloriAmpRpoly(A) signalpause site

pBiT2.1-N[TK-SmBiT-Strep-TagII] vector Sequence

LOCUS       .                       3402 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        28..779
                     /label="HSV TK promoter"
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             893..916
                     /label="Strep-Tag II"
                     /note="peptide that binds Strep-Tactin®, an engineered form
                     of streptavidin"
     polyA_signal    complement(956..1077)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1496..2084)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2287..3144)
                     /label="AmpR"
                     /note="β-lactamase"
     polyA_signal    3249..3297
                     /label="poly(A) signal"
                     /note="synthetic polyadenylation signal"
     misc_feature    3311..3402
                     /label="pause site"
                     /note="RNA polymerase II transcriptional pause signal from
                     the human α2 globin gene"

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