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pT7-7 asyn WT vector (V017885)

Basic Vector Information

Vector Name:
pT7-7 asyn WT
Antibiotic Resistance:
Ampicillin
Length:
2914 bp
Type:
Protein expression
Replication origin:
ori
Host:
E. coli
Copy Number:
Low
Promoter:
T7
5' Primer:
T7
Growth Strain(s):
DH5a
Growth Temperature:
37℃
Expression Method:
IPTG induction

pT7-7 asyn WT vector Map

Copy Sequence View Map
pT7-7 asyn WT2914 bp600120018002400RBST7 promoteroriAmpR promoterAmpR

pT7-7 asyn WT vector Sequence

LOCUS       .                       2914 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     RBS             complement(521..543)
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        complement(575..593)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(1104..1692)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        1908..2012
                     /label="AmpR promoter"
     CDS             2013..2870
                     /label="AmpR"
                     /note="β-lactamase"

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