Basic Vector Information
- Vector Name:
- AAV-pMPRA1-GA
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5896 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells, Adeno-associated virus
- 3' Primer:
- SV40-R2
- Growth Strain(s):
- DB3.1
- Growth Temperature:
- 30℃
AAV-pMPRA1-GA vector Map
AAV-pMPRA1-GA vector Sequence
LOCUS . 5896 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
repeat_region complement(1..141)
/label="AAV2 ITR"
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
protein_bind 148..168
/label="attB4"
/note="core recombination site for the Gateway® BP
reaction"
polyA_signal 238..286
/label="poly(A) signal"
/note="synthetic polyadenylation signal"
misc_feature 300..391
/label="pause site"
/note="RNA polymerase II transcriptional pause signal from
the human α2 globin gene"
protein_bind 1137..1261
/label="attR1"
/note="recombination site for the Gateway® LR reaction"
promoter 1298..1328
/label="lac UV5 promoter"
/note="E. coli lac promoter with an 'up' mutation"
CDS 1382..2038
/label="CmR"
/note="chloramphenicol acetyltransferase"
CDS 2383..2685
/label="ccdB"
/note="CcdB, a bacterial toxin that poisons DNA gyrase"
protein_bind complement(2729..2853)
/label="attR2"
/note="recombination site for the Gateway® LR reaction"
polyA_signal complement(2911..3032)
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
repeat_region 3159..3299
/label="AAV2 ITR"
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 3374..3829
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4111..4215
/label="AmpR promoter"
CDS 4216..5073
/label="AmpR"
/note="β-lactamase"
rep_origin 5247..5835
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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