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pSPT18-T7-5UTR-Fluc-3UTR-BoxCD-polyA vector (V017867)

Basic Vector Information

Vector Name:
pSPT18-T7-5UTR-Fluc-3UTR-BoxCD-polyA
Antibiotic Resistance:
Ampicillin
Length:
5107 bp
Type:
Gene template
Replication origin:
ori
Host:
E. coli
Promoter:
T7
5' Primer:
Forward(left ITR)
Growth Strain(s):
DH5a
Growth Temperature:
37℃

pSPT18-T7-5UTR-Fluc-3UTR-BoxCD-polyA vector Map

Copy Sequence View Map
pSPT18-T7-5UTR-Fluc-3UTR-BoxCD-polyA5107 bp6001200180024003000360042004800SP6 promoterAmpR promoterAmpRoribomluciferase

pSPT18-T7-5UTR-Fluc-3UTR-BoxCD-polyA vector Sequence

LOCUS       .                       5107 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        complement(join(5106..5107,1..17))
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        805..909
                     /label="AmpR promoter"
     CDS             910..1767
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      1941..2529
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(2715..2857)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             3094..4743
                     /label="luciferase"
                     /note="firefly luciferase"

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