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pCfB8644 vector (V017864)

Basic Vector Information

Vector Name:
pCfB8644
Antibiotic Resistance:
Ampicillin
Length:
10708 bp
Type:
Protein expression
Replication origin:
ori
Host:
Yeast
3' Primer:
AmpNR
Growth Strain(s):
DH5a
Growth Temperature:
37℃

pCfB8644 vector Map

Copy Sequence View Map
pCfB864410708 bp5001000150020002500300035004000450050005500600065007000750080008500900095001000010500FRT (minimal)crtYBFRT (minimal)oriAmpR

pCfB8644 vector Sequence

LOCUS       .                      10708 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     protein_bind    1..34
                     /label="FRT (minimal)"
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     CDS             complement(1523..3541)
                     /gene="crtYB"
                     /label="crtYB"
                     /note="Bifunctional lycopene cyclase/phytoene synthase from
                     Phaffia rhodozyma. Accession#: Q7Z859"
     protein_bind    complement(7965..7998)
                     /label="FRT (minimal)"
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     rep_origin      complement(8754..9342)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9467..10324)
                     /label="AmpR"
                     /note="β-lactamase"

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