Basic Vector Information
- Vector Name:
- pMJ920
- Antibiotic Resistance:
- Ampicillin
- Length:
- 10357 bp
- Type:
- Gene-editing vector
- Replication origin:
- ori
- Host:
- Mammalian cells
- Selection Marker:
- Neo/G418
- Promoter:
- CMV
- 5' Primer:
- CMV-F
- Growth Strain(s):
- Mach1
- Growth Temperature:
- 37℃
pMJ920 vector Map
pMJ920 vector Sequence
LOCUS . 10357 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
enhancer 235..614
/label="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 863..881
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 935..5038
/label="Cas9"
/note="S. pyogenes Cas9, codon optimized for C. elegans
with synthetic introns"
CDS 5174..5887
/label="superfolder GFP"
/note="GFP variant that folds robustly even when fused to
poorly folded proteins (Pédelacq et al., 2006)"
promoter complement(5909..5927)
/label="SP6 promoter"
/note="promoter for bacteriophage SP6 RNA polymerase"
polyA_signal 5953..6177
/label="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
rep_origin 6223..6651
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 6665..6995
/label="SV40 promoter"
/note="SV40 enhancer and early promoter"
CDS 7062..7853
/label="NeoR/KanR"
/note="aminoglycoside phosphotransferase"
polyA_signal 8030..8163
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
promoter complement(8248..8278)
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind complement(8293..8314)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(8602..9190)
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(9364..10221)
/label="AmpR"
/note="β-lactamase"
promoter complement(10222..10326)
/label="AmpR promoter"
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