pPLAC8(pig)-Fluc vector (V017856)

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Basic Vector Information

Vector Name:: pPLAC8(pig)-Fluc

Antibiotic Resistance:: Ampicillin

Length:: 6818 bp

Type:: Promoter probe vector

Replication origin:: ori

Host:: Mammalian cells

5' Primer:: RVP3

Growth Strain(s):: DH5a

Growth Temperature:: 37℃

pPLAC8(pig)-Fluc vector Map

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pPLAC8(pig)-Fluc vector Sequence

LOCUS       .                       6818 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             2088..3737
                     /label="luciferase"
                     /note="firefly luciferase"
     polyA_signal    complement(3781..3902)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4321..4909)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5083..5940)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(5941..6045)
                     /label="AmpR promoter"
     rep_origin      6072..6527
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    6658..6706
                     /label="poly(A) signal"
                     /note="synthetic polyadenylation signal"
     misc_feature    6720..6811
                     /label="pause site"
                     /note="RNA polymerase II transcriptional pause signal from
                     the human α2 globin gene"

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