Basic Vector Information
- Vector Name:
- pCCL20-TA-Fluc-SV40-Neo
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4864 bp
- Replication origin:
- ori
- Host:
- Mammalian cells
- Selection Marker:
- Neo/G418
- 5' Primer:
- Fluc
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pCCL20-TA-Fluc-SV40-Neo vector Map
pCCL20-TA-Fluc-SV40-Neo vector Sequence
LOCUS . 4864 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
promoter 67..98
/label="minP"
/note="minimal TATA-box promoter with low basal activity"
CDS 134..1783
/label="luciferase"
/note="firefly luciferase"
polyA_signal complement(1827..1948)
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(2367..2955)
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3129..3986)
/label="AmpR"
/note="β-lactamase"
promoter complement(3987..4091)
/label="AmpR promoter"
rep_origin 4118..4573
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
polyA_signal 4704..4752
/label="poly(A) signal"
/note="synthetic polyadenylation signal"
misc_feature 4766..4857
/label="pause site"
/note="RNA polymerase II transcriptional pause signal from
the human α2 globin gene"
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