Basic Vector Information
- Vector Name:
- pCAGGS-EBFP
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5497 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells
- Promoter:
- CAG
- 5' Primer:
- pcaggs-F
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pCAGGS-EBFP vector Map
pCAGGS-EBFP vector Sequence
LOCUS . 5497 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
intron 663..1680
/label="chimeric intron"
/note="chimera between introns from chicken β-actin and
rabbit β-globin"
CDS 1762..2478
/label="EBFP"
/note="enhanced blue variant of GFP"
polyA_signal 2585..2640
/label="β-globin poly(A) signal"
/note="rabbit β-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind complement(3001..3017)
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(3025..3041)
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG)."
promoter complement(3049..3079)
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind complement(3093..3114)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 3172..3368
/label="SV40 promoter"
/note="SV40 early promoter"
polyA_signal 3374..3508
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(3746..4334)
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4508..5365)
/label="AmpR"
/note="β-lactamase"
promoter complement(5366..5470)
/label="AmpR promoter"
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