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prCO vector (V017837)

Basic Vector Information

Vector Name:
prCO
Antibiotic Resistance:
Ampicillin
Length:
7315 bp
Type:
Protein expression
Replication origin:
ori
Host:
Mammalian cells, Retrovirus
Selection Marker:
Neo/G418
Promoter:
LTR
5' Primer:
PLXSN-F
Growth Strain(s):
Stbl3
Growth Temperature:
37℃

prCO vector Map

Copy Sequence View Map
prCO7315 bp300600900120015001800210024002700300033003600390042004500480051005400570060006300660069007200LTRMMLV Ψgag (truncated)SV40 NLSSV40 NLSchimeric intronIRES2NeoR/KanRLTRoriAmpRAmpR promoter

prCO vector Sequence

LOCUS       .                       7315 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             8..477
                     /label="LTR"
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     misc_feature    542..741
                     /label="MMLV Ψ"
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             942..1358
                     /label="gag (truncated)"
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
     CDS             1423..1443
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             1465..1485
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     intron          2278..2507
                     /label="chimeric intron"
                     /note="chimera between introns from adenovirus and
                     immunoglobulin heavy chain genes"
     misc_feature    2580..3166
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             3194..3994
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase from Tn5"
     LTR             4687..5278
                     /label="LTR"
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     rep_origin      complement(5579..6167)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6330..7187)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(7188..7292)
                     /label="AmpR promoter"

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