pLEX_305-N-dTAG vector (V017832)

LOCUS       .                       9650 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        13..516
                     /label="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             525..848
                     /label="FKBP-V"
                     /note="F36V mutant of FK506-binding protein FKBP12"
     CDS             855..881
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             885..911
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     protein_bind    927..1051
                     /label="attR1"
                     /note="recombination site for the Gateway® LR reaction"
     promoter        1076..1106
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1160..1816
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     CDS             2161..2463
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(2507..2631)
                     /label="attR2"
                     /note="recombination site for the Gateway® LR reaction"
     misc_feature    2683..3271
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             3343..3576
                     /label="3' LTR (ΔU3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3654..3788
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(3971..3989)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3999..4015)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      4157..4612
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4638..4742
                     /label="AmpR promoter"
     CDS             4743..5600
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      5774..6362
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    6650..6671
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6686..6716
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    6724..6740
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     primer_bind     6748..6764
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        6785..6803
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6831..7057
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     misc_feature    7285..7410
                     /label="HIV-1 Ψ"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    7903..8136
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             8321..8365
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     promoter        8596..8806
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     CDS             8846..9442
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    9491..9608
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"