pLEX_305-C-dTAG vector (V017831)

LOCUS       .                       9621 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     polyA_signal    221..355
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      382..517
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(538..556)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(566..582)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      724..1179
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1205..1309
                     /label="AmpR promoter"
     CDS             1310..2167
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      2341..2929
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    3217..3238
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3253..3283
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    3291..3307
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     primer_bind     3315..3331
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        3352..3370
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3398..3624
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3625..3805
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3852..3977
                     /label="HIV-1 Ψ"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    4470..4703
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             4888..4932
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     promoter        5163..5373
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     CDS             5413..6009
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    6058..6175
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        6230..6733
                     /label="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
     protein_bind    6754..6878
                     /label="attR1"
                     /note="recombination site for the Gateway® LR reaction"
     promoter        6903..6933
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             6987..7643
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     CDS             7988..8290
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(8334..8458)
                     /label="attR2"
                     /note="recombination site for the Gateway® LR reaction"
     CDS             8466..8786
                     /label="DmrB"
                     /note="F36V mutant of FK506-binding protein FKBP12"
     CDS             8793..8819
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             8823..8849
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     misc_feature    8871..9459
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"