pHCMV-Env-emiRFP670 vector (V017829)

LOCUS       .                       7749 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        113..492
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        493..696
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          808..1380
                     /label="β-globin intron"
                     /note="intron from rabbit β-globin gene"
     CDS             2398..3327
                     /label="emiRFP670"
                     /note="emiRFP670 is a basic (constitutively fluorescent)
                     near ir fluorescent protein published in 2020, derived from
                     Rhodopseudomonas palustris. It has low acid sensitivity. It
                     requires the cofactor biliverdin for fluorescence."
     polyA_signal    4535..4590
                     /label="β-globin poly(A) signal"
                     /note="rabbit β-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(4948..4964)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4972..4988)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4996..5026)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5041..5062)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5350..5938)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6112..6969)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(6970..7074)
                     /label="AmpR promoter"
     rep_origin      complement(7100..7555)
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     7697..7713
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        7723..7741
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"