Basic Vector Information
- Vector Name:
- LATS2-P2A-ECFP-YAP1
- Antibiotic Resistance:
- Ampicillin
- Length:
- 10961 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells
- Selection Marker:
- Neo/G418
- Promoter:
- CMV
- 5' Primer:
- CMV-F
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
LATS2-P2A-ECFP-YAP1 vector Map
LATS2-P2A-ECFP-YAP1 vector Sequence
LOCUS . 10961 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
enhancer 202..581
/label="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 582..785
/label="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 830..848
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 4241..4957
/label="ECFP"
/note="enhanced CFP"
CDS 4964..6475
/gene="YAP1"
/label="YAP1"
/note="Transcriptional coactivator YAP1 from Homo sapiens.
Accession#: P46937"
polyA_signal 6528..6752
/label="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
rep_origin 6798..7226
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 7240..7569
/label="SV40 promoter"
/note="SV40 enhancer and early promoter"
CDS 7636..8427
/label="NeoR/KanR"
/note="aminoglycoside phosphotransferase"
polyA_signal 8604..8737
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
promoter complement(8822..8852)
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind complement(8867..8888)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(9176..9761)
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(9935..10792)
/label="AmpR"
/note="β-lactamase"
promoter complement(10793..10897)
/label="AmpR promoter"
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