Basic Vector Information
- Vector Name:
- pQE80L TriCatcher-RGDSP
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6539 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- E. coli
- Copy Number:
- Low
- Promoter:
- T5
- 5' Primer:
- pQE30-F
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
- Expression Method:
- IPTG induction
pQE80L TriCatcher-RGDSP vector Map
pQE80L TriCatcher-RGDSP vector Sequence
LOCUS . 6539 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
promoter 82..126
/label="T5 promoter"
/note="bacteriophage T5 promoter for E. coli RNA
polymerase, with embedded lac operator"
CDS 202..219
/label="6xHis"
/note="6xHis affinity tag"
CDS 238..258
/label="TEV site"
/note="tobacco etch virus (TEV) protease recognition and
cleavage site"
CDS 1198..1218
/label="TEV site"
/note="tobacco etch virus (TEV) protease recognition and
cleavage site"
terminator 2068..2162
/label="lambda t0 terminator"
/note="transcription terminator from phage lambda"
CDS 2206..2862
/label="CmR"
/note="chloramphenicol acetyltransferase"
terminator 2930..3016
/label="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
protein_bind complement(3075..3096)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
CDS complement(3112..4191)
/label="lacI"
/note="lac repressor"
promoter complement(4192..4269)
/label="lacIq promoter"
/note="In the lacIq allele, a single base change in the
promoter boosts expression of the lacI gene about 10-fold."
misc_feature 4461..4601
/label="bom"
/note="basis of mobility region from pBR322"
rep_origin complement(4787..5375)
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5549..6406)
/label="AmpR"
/note="β-lactamase"
promoter complement(6407..6511)
/label="AmpR promoter"
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