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pPSU150 vector (V017801)

Basic Vector Information

Vector Name:
pPSU150
Antibiotic Resistance:
Ampicillin
Length:
6808 bp
Type:
Protein expression
Replication origin:
ori
Host:
E. coli
Copy Number:
Low
Promoter:
T7
5' Primer:
T7
Growth Strain(s):
DH5a
Growth Temperature:
37℃

pPSU150 vector Map

Copy Sequence View Map
pPSU1506808 bp3006009001200150018002100240027003000330036003900420045004800510054005700600063006600T7 promoterRBSStrep-Tag II9xHisProtAMBPT7 terminatorAmpR promoterAmpRfd terminatororibomrop

pPSU150 vector Sequence

LOCUS       .                       6808 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        27..45
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             113..135
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             152..175
                     /label="Strep-Tag II"
                     /note="peptide that binds Strep-Tactin®, an engineered form
                     of streptavidin"
     CDS             185..211
                     /label="9xHis"
                     /note="9xHis affinity tag"
     CDS             236..406
                     /label="ProtA"
                     /note="IgG-binding unit of Staphylococcus aureus protein A"
     CDS             413..1510
                     /label="MBP"
                     /note="maltose binding protein from E. coli"
     terminator      4254..4301
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     promoter        4366..4457
                     /label="AmpR promoter"
     CDS             4458..5315
                     /label="AmpR"
                     /note="β-lactamase"
     terminator      5322..5361
                     /label="fd terminator"
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      5392..5980
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(6166..6305)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(6410..6598)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"

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