Basic Vector Information
- Vector Name:
- pBS598 EF1alpha-EGFPcre
- Antibiotic Resistance:
- Ampicillin
- Length:
- 8178 bp
- Type:
- Protein expression, Cre
- Replication origin:
- ori
- Host:
- Mammalian cells
- Promoter:
- EF1a
- 5' Primer:
- pEGFP-C-5'
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pBS598 EF1alpha-EGFPcre vector Map
pBS598 EF1alpha-EGFPcre vector Sequence
LOCUS . 8178 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
primer_bind 1..17
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
promoter 107..1285
/label="EF-1α promoter"
/note="strong constitutive promoter for human elongation
factor EF-1α"
CDS 1315..2031
/label="EGFP"
/note="the original enhanced GFP (Yang et al., 1996)"
CDS 2086..3114
/label="Cre"
/note="site-specific recombinase"
primer_bind complement(5579..5595)
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(5603..5619)
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG)."
promoter complement(5627..5657)
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind complement(5672..5693)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(5981..6569)
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(6743..7600)
/label="AmpR"
/note="β-lactamase"
promoter complement(7601..7705)
/label="AmpR promoter"
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