Basic Vector Information
- Vector Name:
- pJRH-1179 U6-reci Gag-Cas9 v2
- Antibiotic Resistance:
- Ampicillin
- Length:
- 11206 bp
- Type:
- Gene-editing vector
- Replication origin:
- ori
- Host:
- Mammalian cells
- Promoter:
- CAG,U6
- 5' Primer:
- U6-F
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pJRH-1179 U6-reci Gag-Cas9 v2 vector Map
pJRH-1179 U6-reci Gag-Cas9 v2 vector Sequence
LOCUS . 11206 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
promoter 82..322
/label="U6 promoter"
/note="RNA polymerase III promoter for human U6 snRNA"
intron 1150..2166
/label="chimeric intron"
/note="chimera between introns from chicken β-actin and
rabbit β-globin"
CDS 2227..3726
/label="HIV-1 gag"
/note="gag protein from human immunodeficiency virus 1"
CDS 3727..3753
/label="NES"
/note="nuclear export signal from the HIV Rev protein
(Fischer et al., 1995)"
CDS 3766..3792
/label="NES"
/note="nuclear export signal from the HIV Rev protein
(Fischer et al., 1995)"
CDS 3805..3831
/label="NES"
/note="nuclear export signal from the HIV Rev protein
(Fischer et al., 1995)"
CDS 4003..8106
/label="Cas9"
/note="S. pyogenes Cas9, codon optimized for C. elegans
with synthetic introns"
CDS 8110..8175
/label="3xFLAG"
/note="three tandem FLAG® epitope tags, followed by an
enterokinase cleavage site"
CDS 8194..8214
/label="SV40 NLS"
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
CDS 8221..8241
/label="SV40 NLS"
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
polyA_signal 8319..8374
/label="β-globin poly(A) signal"
/note="rabbit β-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
promoter complement(8783..8813)
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind complement(8828..8849)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 8907..9103
/label="SV40 promoter"
/note="SV40 early promoter"
polyA_signal 9109..9243
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(9482..10070)
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(10244..11101)
/label="AmpR"
/note="β-lactamase"
promoter complement(11102..11206)
/label="AmpR promoter"
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