pLenti-PGK-Neo-PIP-FUCCI vector (V017796)

LOCUS       .                      10145 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..181
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label="HIV-1 Ψ"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    846..1079
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1264..1308
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1457..1498
                     /gene="Protein Tat"
                     /label="Protein Tat"
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     misc_feature    1575..1692
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        1738..2248
                     /label="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
     protein_bind    2303..2327
                     /label="attB1"
                     /note="recombination site for the Gateway® BP reaction"
     CDS             2399..2419
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             2429..2455
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             2459..2485
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             2492..3205
                     /label="Venus"
                     /note="yellow fluorescent protein (YFP) with fast and
                     efficient maturation (Nagai et al., 2002)"
     CDS             3284..3991
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     protein_bind    complement(4344..4368)
                     /label="attB2"
                     /note="recombination site for the Gateway® BP reaction"
     misc_feature    4399..4987
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     promoter        5030..5529
                     /label="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             5543..6343
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase from Tn5"
     LTR             6437..6670
                     /label="3' LTR (ΔU3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6742..6876
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      6903..7038
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(7059..7077)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      7245..7700
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        7726..7830
                     /label="AmpR promoter"
     CDS             7831..8688
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      8862..9450
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    9738..9759
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9774..9804
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     promoter        9873..9891
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"