pAAVS1-PC-GCaMP6f vector (V017790)

LOCUS       .                       8007 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    1..804
                     /label="HA-L"
                     /note="left homology arm from the adeno-associated virus
                     integration site (AAVS1) within intron 1 of the human
                     PPP1R12C gene"
     misc_feature    846..871
                     /label="SA"
                     /note="splice acceptor site"
     CDS             895..948
                     /label="T2A"
                     /note="2A peptide from Thosea asigna virus capsid protein"
     CDS             958..1554
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     polyA_signal    1595..1819
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     intron          2485..3502
                     /label="chimeric intron"
                     /note="chimera between introns from chicken β-actin and
                     rabbit β-globin"
     CDS             3664..3915
                     /label="VC155"
                     /note="C-terminal fragment of mVenus for use in bimolecular
                     fluorescence complementation (BiFC) (Kodama and Hu, 2010)"
     polyA_signal    4973..5028
                     /label="β-globin poly(A) signal"
                     /note="rabbit β-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     protein_bind    complement(5377..5410)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5451..6287
                     /label="HA-R"
                     /note="right homology arm from the adeno-associated virus
                     integration site (AAVS1) within intron 1 of the human
                     PPP1R12C gene"
     rep_origin      complement(6335..6923)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7097..7954)
                     /label="AmpR"
                     /note="β-lactamase"