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pBAD18-EGFP vector (V017789)

Basic Vector Information

Vector Name:
pBAD18-EGFP
Antibiotic Resistance:
Ampicillin
Length:
5315 bp
Type:
Protein expression
Replication origin:
ori
Host:
E. coli
Copy Number:
Low
Promoter:
BAD
5' Primer:
PBAD30.F
Growth Strain(s):
DH5a
Growth Temperature:
37℃
Expression Method:
Arabinose induction

pBAD18-EGFP vector Map

Copy Sequence View Map
pBAD18-EGFP5315 bp6001200180024003000360042004800araCaraBAD promoterEGFPrrnB T1 terminatorrrnB T2 terminatorAmpR promoterAmpRf1 orioribom

pBAD18-EGFP vector Sequence

LOCUS       .                       5315 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             complement(99..974)
                     /label="araC"
                     /note="L-arabinose regulatory protein"
     promoter        1001..1285
                     /label="araBAD promoter"
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite
                     direction (Guzman et al., 1995)"
     CDS             1339..2055
                     /label="EGFP"
                     /note="the original enhanced GFP (Yang et al., 1996)"
     terminator      2267..2353
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2445..2472
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        2491..2582
                     /label="AmpR promoter"
     CDS             2583..3440
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      3485..3940
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      4051..4639
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(4825..4965)
                     /label="bom"
                     /note="basis of mobility region from pBR322"

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