Basic Vector Information
- Vector Name:
- pROS16
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5465 bp
- Type:
- Gene-editing vector
- Replication origin:
- ori
- Host:
- Yeast
- Selection Marker:
- HIS3
- Promoter:
- SNR52
- 3' Primer:
- AmpNR
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pROS16 vector Map
pROS16 vector Sequence
LOCUS . 5465 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
promoter complement(join(5415..5465,1..218))
/label="SNR52 promoter"
/note="promoter for the S. cerevisiae small nucleolar RNA
gene SNR52"
rep_origin complement(522..1110)
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1284..2141)
/label="AmpR"
/note="β-lactamase"
promoter complement(2142..2246)
/label="AmpR promoter"
promoter 2483..2669
/label="HIS3 promoter"
CDS 2670..3329
/label="HIS3"
/note="imidazoleglycerol-phosphate dehydratase, required
for histidine biosynthesis"
promoter 3657..3925
/label="SNR52 promoter"
/note="promoter for the S. cerevisiae small nucleolar RNA
gene SNR52"
misc_RNA 3946..4020
/label="gRNA scaffold"
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
terminator 4025..4044
/label="SUP4 terminator"
/note="transcription terminator for the S. cerevisiae SUP4
tRNA gene"
rep_origin 4090..5250
/label="2μ ori"
/note="yeast 2μ plasmid origin of replication"
terminator complement(5296..5315)
/label="SUP4 terminator"
/note="transcription terminator for the S. cerevisiae SUP4
tRNA gene"
misc_RNA complement(5320..5394)
/label="gRNA scaffold"
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
This page is informational only.