Basic Vector Information
- Vector Name:
- pET-32M
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5806 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- E. coli
- Copy Number:
- Low
- Promoter:
- T7
- 5' Primer:
- T7
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
- Expression Method:
- IPTG induction
pET-32M vector Map
pET-32M vector Sequence
LOCUS . 5806 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
promoter 1..19
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 20..44
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG)."
RBS 59..81
/label="RBS"
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 89..415
/label="TrxA"
/note="E. coli thioredoxin"
CDS 437..454
/label="6xHis"
/note="6xHis affinity tag"
CDS 464..487
/label="HRV 3C site"
/note="recognition and cleavage site for human rhinovirus
3C and PreScission proteases"
CDS 534..551
/label="6xHis"
/note="6xHis affinity tag"
terminator 618..665
/label="T7 terminator"
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
rep_origin 702..1157
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 1183..1287
/label="AmpR promoter"
CDS 1288..2145
/label="AmpR"
/note="β-lactamase"
rep_origin 2319..2907
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature complement(3093..3232)
/label="bom"
/note="basis of mobility region from pBR322"
CDS complement(3337..3525)
/label="rop"
/note="Rop protein, which maintains plasmids at low copy
number"
protein_bind complement(4300..4321)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
CDS complement(4337..5416)
/label="lacI"
/note="lac repressor"
promoter complement(5417..5494)
/label="lacI promoter"
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