Basic Vector Information
- Vector Name:
- pGAG
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6423 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells
- Promoter:
- CAG
- 5' Primer:
- pcaggs-F
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pGAG vector Map
pGAG vector Sequence
LOCUS . 6423 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
enhancer 73..376
/label="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
intron 654..1670
/label="chimeric intron"
/note="chimera between introns from chicken β-actin and
rabbit β-globin"
CDS 1776..3275
/label="HIV-1 gag"
/note="gag protein from human immunodeficiency virus 1"
CDS 3276..3302
/label="NES"
/note="nuclear export signal from the HIV Rev protein
(Fischer et al., 1995)"
CDS 3315..3341
/label="NES"
/note="nuclear export signal from the HIV Rev protein
(Fischer et al., 1995)"
CDS 3354..3380
/label="NES"
/note="nuclear export signal from the HIV Rev protein
(Fischer et al., 1995)"
polyA_signal 3502..3557
/label="β-globin poly(A) signal"
/note="rabbit β-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind complement(3918..3934)
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(3942..3958)
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG)."
promoter complement(3966..3996)
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind complement(4011..4032)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 4090..4286
/label="SV40 promoter"
/note="SV40 early promoter"
polyA_signal 4292..4426
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(4665..5253)
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5427..6284)
/label="AmpR"
/note="β-lactamase"
promoter complement(6285..6389)
/label="AmpR promoter"
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