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pLXSN18E6E7 vector (V017770)

Basic Vector Information

Vector Name:
pLXSN18E6E7
Antibiotic Resistance:
Ampicillin
Length:
6688 bp
Type:
Protein expression
Replication origin:
ori
Host:
Mammalian cells, Retrovirus
Selection Marker:
Neo/G418
Copy Number:
Low
5' Primer:
PLXSN-F
Growth Strain(s):
Stbl3
Growth Temperature:
37℃

pLXSN18E6E7 vector Map

Copy Sequence View Map
pLXSN18E6E76688 bp30060090012001500180021002400270030003300360039004200450048005100540057006000630066005' LTRMMLV Ψgag (truncated)SV40 promoterNeoR/KanRLTRbomoriAmpRAmpR promoter

pLXSN18E6E7 vector Sequence

LOCUS       .                       6688 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             176..763
                     /label="5' LTR"
                     /note="long terminal repeat from Moloney murine sarcoma
                     virus"
     misc_feature    826..1025
                     /label="MMLV Ψ"
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             1226..1642
                     /label="gag (truncated)"
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
     promoter        2491..2820
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             2880..3671
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     LTR             3734..4327
                     /label="LTR"
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     misc_feature    4538..4678
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4864..5452)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5626..6483)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(6484..6588)
                     /label="AmpR promoter"

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