Basic Vector Information
- Vector Name:
- pROS13
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5809 bp
- Type:
- Gene-editing vector
- Replication origin:
- ori
- Host:
- Yeast
- Selection Marker:
- Neo/G418
- Promoter:
- SNR52
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pROS13 vector Map
pROS13 vector Sequence
LOCUS . 5809 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
protein_bind 18..51
/label="loxP"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
gene 106..1462
/label="kanMX"
/note="yeast selectable marker conferring kanamycin
resistance (Wach et al., 1994)"
promoter 1681..1949
/label="SNR52 promoter"
/note="promoter for the S. cerevisiae small nucleolar RNA
gene SNR52"
misc_RNA 1970..2044
/label="gRNA scaffold"
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
terminator 2049..2068
/label="SUP4 terminator"
/note="transcription terminator for the S. cerevisiae SUP4
tRNA gene"
rep_origin 2114..3274
/label="2μ ori"
/note="yeast 2μ plasmid origin of replication"
terminator complement(3320..3339)
/label="SUP4 terminator"
/note="transcription terminator for the S. cerevisiae SUP4
tRNA gene"
misc_RNA complement(3344..3418)
/label="gRNA scaffold"
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
promoter complement(3439..3707)
/label="SNR52 promoter"
/note="promoter for the S. cerevisiae small nucleolar RNA
gene SNR52"
rep_origin complement(4011..4599)
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4773..5630)
/label="AmpR"
/note="β-lactamase"
promoter complement(5631..5735)
/label="AmpR promoter"
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