pAAV-hSyn-MCS-PGK-EGFP-E2A-Puro vector (V017740)

LOCUS       .                       5877 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     repeat_region   complement(1..141)
                     /label="AAV2 ITR"
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
     promoter        186..633
                     /label="hSyn promoter"
                     /note="human synapsin I promoter; confers neuron-specific
                     expression (Kügler et al., 2003)"
     promoter        670..1169
                     /label="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             1196..1912
                     /label="EGFP"
                     /note="the original enhanced GFP (Yang et al., 1996)"
     polyA_signal    2624..3100
                     /label="hGH poly(A) signal"
                     /note="human growth hormone polyadenylation signal"
     repeat_region   3140..3280
                     /label="AAV2 ITR"
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
     rep_origin      3355..3810
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4092..4196
                     /label="AmpR promoter"
     CDS             4197..5054
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      5228..5816
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"