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phPGK-Puro-P2A-mTagBFP2 vector (V017739)

Basic Vector Information

Vector Name:
phPGK-Puro-P2A-mTagBFP2
Antibiotic Resistance:
Ampicillin
Length:
4416 bp
Type:
Protein expression
Replication origin:
ori
Host:
Mammalian cells
Selection Marker:
Puro
5' Primer:
hPGK-F
Growth Strain(s):
DH5a
Growth Temperature:
37℃

phPGK-Puro-P2A-mTagBFP2 vector Map

Copy Sequence View Map
phPGK-Puro-P2A-mTagBFP24416 bp600120018002400300036004200M13 fwdhPGK promoterPuroRmTagBFP2SV40 NLSoriAmpRAmpR promoter

phPGK-Puro-P2A-mTagBFP2 vector Sequence

LOCUS       .                       4416 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        478..985
                     /label="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             1007..1603
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     CDS             1685..2395
                     /label="mTagBFP2"
                     /note="enhanced monomeric blue fluorescent protein (Subach
                     et al., 2011)"
     CDS             2396..2416
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     rep_origin      complement(2597..3185)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3359..4216)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(4217..4321)
                     /label="AmpR promoter"

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